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Fig. 6 | BMC Plant Biology

Fig. 6

From: TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici

Fig. 6

Silencing of TaClpS1 hinders the expansion of Pst on wheat leaves. a Wheat germ agglutinin (WGA) was used to stain fungal structures in wheat leaves infected with BSMV and Pst. The fungal structures were observed under an autofluorescence microscope. SV, sub-stomatal vesicle; IH, infection hypha; H, haustoria; HMC, haustorial mother cell. b The haustoria number per infection site was counted in BSMV-infected wheat leaves inoculated with CYR23 at 24 hpi. c The length of IH was measured in BSMV-infected leaves inoculated with CYR23 at 24 hpi. Hyphal length is the distance from the intersection of the sub-stomatal vesicle and the hypha to the tip of the hypha, which was measured by DP-BSW (units in μm) program. d The infection area was measured in BSMV-infected leaves inoculated with CYR23 at 120 hpi using DP-BSW program (units in μm2). Results shown here were derived from three biological replicates and each replication included 40 individual infection sites. Each data denote the means ± standard deviation of three biological replicates. Asterisks (P < 0.05) or double asterisks (P < 0.01) indicate significant differences from BSMV: γ treatment at the same time points calculated by unpaired two-tailed Student’s t-test

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