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Fig. 4 | BMC Plant Biology

Fig. 4

From: TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici

Fig. 4

BSMV-mediated silencing of TaClpS1 enhances wheat resistance to Pst. a Symptoms of BSMV-infected wheat leaves, and phenotypes of the fourth leaves of silenced plants inoculated with Pst isolateCYR23 at 12 dpi. b-d Relative transcript levels of TaClpS1 (b) and the pathogenesis-related genesTaPR1 (c) and TaPR2 (d) in TaClpS1-silenced leaves infected with Pst isolate CYR23. The comparative threshold (2-ΔΔCT) approach was used to measure the relative transcript levels of TaClpS1, TaPR1 and TaPR2. Data were normalized with the transcription level of TaEF-1α, and visualized as the fold changes compared to control at 0 hpi. BSMV: γ infected wheat leaves were used as a control. Each data point represents the means ± standard deviation of three independent biological replicates. Single asterisks (P < 0.05) and double asterisks (P < 0.01) denote significant differences from BSMV: γ treatment calculated by unpaired two-tailed Student’s t-test

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