Fig. 2
From: TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici
Subcellular localization of TaClpS1 in Nicotiana benthamiana and wheat protoplasts. a Subcellular localization of TaClpS1 in Nicotiana benthamiana. pCAMBIA1302: GFP, pCAMBIA1302: TaClpS1–GFP or pCAMBIA1302: TaClpS1Δ–GFP constructs were transiently overexpressed in N. benthamiana leaves using agroinfiltration. Olympus IX83 confocal microscope was used to photograph GFP signals at 48 h after agroinfiltration. Bar, 50 μm. b Subcellular localization of TaClpS1 in wheat protoplasts. The pCaMV35S: TaClpS1-GFP or pCaMV35S: TaClpS1Δ-GFP or pCaMV35S: GFP constructs were independently overexpressed in wheat protoplasts by PEG-mediated transformation. Bar, 20 μm