Fig. 2

Defense response of MS plants to B. cinerea and A. brassicicola infection. The 5th to 7th true leaf from 17-day old plants were inoculated with 5 μL of 3.5 × 10⁴ spores/mL^− 1 of pathogens (n = 15 plants). A Lactophenol-trypan blue was used to stain fungal hyphae and to determine necrotic lesion denoted by circle in leaves from control and MS plants 48 hpi. A representative stained image and the average lesion area are displayed (n = 15 leaves). B Representative images of leaves from MS wild-type plant (WT) and the JA mutant coi1 infected with A. brassicicola (5 dpi). C Necrotic lesions were stained with lactophenol trypan blue and the lesion area (mm²) denoted by circle with arrow was measured to provide a quantitative measurement of pathogen proliferation (n = 15). All data are representative of at least two independent experiments. Error bars show the standard error of biological variation. Statistical significance denoted by letters was determined using ANOVA with the Bonferroni test (P < 0.05). Scale bars = A, 0.2 cm B, 1 cm; C, 0.3 cm