Fig. 8

Chloroplast differentiation during regeneration of androgenic plants of ‘Jersey’ and ‘Mercada’ cultivars. a, b, c The relative expression level of nuclear-localised genes encoding: phytochromes A and B (a), transcription factors GLK1 and GLK2 (b) and PIF1 regulating the chlorophyll synthesis (c). d Types and density of plastids observed during regeneration. Graphs show mean values of n ≥ 3 with SEM in a-c and SD in d. Relative expression level was normalised to 43dC of cv. ‘Jersey’. An asterisk presents a value significantly different between cultivars at a certain day of culture. A hash indicates a value significantly different from the preceding day of culture within cultivar (Tukey’s test, P < 0.05). dC – day of culture