Fig. 5

Plastid DNA content during isolated microspore culture of ‘Jersey’ and ‘Mercada’ cultivars. a Average plastome copy number in relation to nuclear genome in subsequent days of isolated microspore culture. b The individual copy number of genes localised in the plastid genome in subsequent days of isolated microspore culture of ‘Jersey’ and ‘Mercada’ cultivars. c The relative expression profile of Polγ (Organellar DNA polymeraseI) gene and DPD1 gene encoding Mg²⁺ -dependent organelle exonuclease. Graphs show mean values of n ≥ 3 with SD in a and b or SEM in c. Relative expression level was normalised to ML microspores of cv. ‘Jersey’. An asterisk presents a value significantly different between cultivars at a certain day of culture. A hash indicates a value significantly different from the preceding day of culture within cultivar (Tukey’s test, P < 0.05). A $ indicates a value significantly different from the calculated average plastome copy number at a particular day of culture within cultivar (Student’s t-test, P < 0.05). Red lines show the average copy numbers calculated from individual copies of presented genes. LSC – long single copy, IR – inverted repeat, SSC – short single copy, ML – mid-to-late microspore, PM – pre-treated microspores, dC – day of culture