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Fig. 6 | BMC Plant Biology

Fig. 6

From: MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Fig. 6

MtNIP5;1 partially complements nip5;1–1 B deficiency phenotype. a Images of seedlings (Wild type (Wt), two independent nip5;1 mutant lines expressing p35S::MtNIP5;1-GFP (p35S::MtNIP5;1-GFP 1 and p35S::MtNIP5;1-GFP 2), and nip5;1–1) growing under control (left panel) or B deficient conditions (right panel). Bar = 1 cm. b Primary root length of Wt (black columns), p35S::MtNIP5;1-GFP 1 (dark grey columns), p35S::MtNIP5;1-GFP 2 (light grey columns), and nip5;1–1 (white columns) was measured 10 days postgermination. c B concentration was quantified in 10-days old Wt (black), p35S::MtNIP5;1-GFP 1 (dark grey), p35S::MtNIP5;1-GFP 2 (light grey), and nip5;1–1 (white) seedlings grown under B control and B deficiency conditions. The two B treatments used were: control (100 μM B [OH3]) and deficiency (0.03 μM B [OH3]). Different letters indicate significant differences using a One-way ANOVA analysis followed by Tukey’s test (p < 0.05)

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