Fig. 5
From: MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency
Inmunolocation of MtNIP5;1 in the root epidermis and nodules of Medicago truncatula. a Subcellular localization by immunostaining of MtNIP5;1-HA with the antibody Alexa 594 was performed in root sections of 4-week-old M. truncatula expressing p35S::MtNIP5;1-HA (red, DsRed channel) under different B conditions (control and deficiency). The three bottom rows show detail of root epidermal and hair root cells showing MtNIP5;1-HA localization. b Subcellular localization by immunostaining of MtNIP5;1-HA with the antibody Alexa 594 was performed in nodule sections of 4-week-old M. truncatula expressing p35S::MtNIP5;1-HA (red, DsRed channel), inoculated with a Sinorhizobium meliloti 2011 strain and growing under different B conditions (control, middle row, and deficiency, bottom row). The three upper rows of images are taken at 10Xmagnification, while the three bottom rows show detail of nodule cortex and fixation zone at 40X magnification, showing MtNIP5;1-HA localization. Left column images show HA signal, middle column shows bright-field images and right column shows the merged images of HA signal and bright field. Upper rows in each panel show autofluorescence controls, in which no anti-HA primary antibody was used. Bars = 100 μm (10X images) or 50 μm (40X images)