Fig. 4
From: MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency
Subcellular localization of MtNIP5;1 in N. benthamiana leaves evidences its localization at the plasma membrane. Left panel shows the GFP signal of MtNIP5;1-GFP, left middle panel shows CFP signal of AtPIP2A-CFP, middle panel shows GFP and CFP merged images, right middle panel shows the bright field image, and right panel the merge between bright-field, GFP signal, and CFP signal. p35S::MtNIP5;1-GFP and p35S::AtPIP2A-CFP were co-transformed into N. benthamiana leaves. The middle row shows N. benthamiana cells transformed with p35S::GFP as a transformation positive control. The bottom row shows the negative control images obtained from non-transformed leaves. Bar = 50 μm