Fig. 2
From: MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency
M. truncatula MtNIP5;1 is the closest homologous protein to the A. thaliana AtNIP5;1, an aquaporin involved in B transport under deficiency. a Unrooted phylogenetic tree of M. truncatula homologs to AtNIP5;1 (which is indicated by a red square) was obtained using Mega7 software. The tree is drawn so that branch length separating any two sequences is proportional to evolutionary distances. b MtNIP5;1 protein predicted model. Upper panel within panel B indicates the tertiary structure model of MtNIP5;1 showing yellow areas that represent the NPS/NPV motifs. Distribution of positive (blue) and negative (red) charges are shown below. The location of positively charged residues in the external part of the pore leaving the negative charges in the inside is shown. c Alignment of AtNIP5;1 and MtNIP5;1 protein sequences. Shaded in grey appear the six transmembranous helices and marked in yellow, the regions that form the seventh helix. The NPA motifs are shown in red, residues from the Ar/R selectivity filter appear in green and the five amino acids that shape the Froger’s positions are shown in blue