Table 2 Primers used for cloning and gene expression studies in the study
From: Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression
Primer | Sequence |
|---|---|
Arabidopsis thaliana | |
AtAGL8qPCR-F | TGCGCTCCAGAAGAAGGATAAAGC |
AtAGL8 qPCR-R | TTCCGTCAACGACGATGCACCA |
AtAHL20CDS-F | ATGGCAAACCCTTGGTGGAC |
AtAHL20CDS-R | TCAGTAAGGTGGTCTTGCGT |
AtAHL20-ATTB-F | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCAAACCCTTGGTGGAC |
AtAHL20-ATTB-Rv | GGGGACCACTTTGTACAAGAAAGCTGGGTCGTAAGGTGGTCTTGCGTGGA |
AtAHL20qPCR-Fw | CGTTGAGGTGGTCAACCGTA |
AtAHL20qPCR-Rv | TTGCCTGCGTCTTGAGAAGT |
AtFTqPCR-F | CCAAGTCCTAGCAACCCTCA |
AtFTqPCR-R | TACACTGTTTGCCTGCCAAG |
AtMDAR4q PCR-Fw | GCGGTGGCTATATCGGTATGG |
AtMDAR4q PCR-Rv | AAAGAGACGTGCCATGCAGTG |
AtSPL3qPCR-F1 | CTTAGCTGGACACAACGAGAGAAGG |
AtSPL3qPCR-R1 | GAGAAACAGACAGAGACACAGAGGA |
AtTSFqPCR-F | GAGTCCAAGCAACCCTCACCAA |
AtTSFqPCR-R | CACCACAATACGATGAATTCCCGAG |
UBQ10 -F | GGCCTTGTATAATCCCTGATGAATAAG |
UBQ10 -R | AAAGAGATAACAGGAACGGAAACATAGT |
Promoter-GUS | |
AtAHL20Prom-ATTB-Fw | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTTGTAGCGGTAAATTGTGGCTTAA |
AtAHL20Prom-ATTB-Rv | GGGGACCACTTTGTACAAGAAAGCTGGGTCGATTGACCAAAAACTGGAAATTCGC |
Camelina sativa | |
CsAHL20-Fw | AACGGTTTACTTAGCCGGGG |
CsAHL20-Rv | GCAGCTATCACCATGACCGA |
CsAHL20-ATTB-Fw | GGGGACAAGTTTGTACAAAAAAGCAGGCTATATGTCAAACCCTTGGTGGACG |
CsAHL20-ATTB-Rv | GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAGTATGGTGGTCGCGCGTG |
CsFT-Fw | AGGAATTCACCGTGTCGTGA |
CsFT-Rv | CGAGTGTTGAAGTTCTGGCG |
CsMDAR4-Fw | TTGGCGAAATGAGGAGGCTT |
CsMDAR4-Rv | AATGCCATGAGAAGGCGAA |