Fig. 2

Phenotypic and flowering-time analysis of 35S:AtAHL20 transgenic plants. a 35S:AtAHL20 transgenic plants displayed dwarf phenotypes compared to the wild type, 35S:AtAHL20m1, 35S:AtAHL20m2, ahl20-1 and ahl20-2 plants under LD conditions. b AtAHL20 expression levels in 35S:AtAHL20 and 35S:AtAHL20m plants. c and d 35S:AtAHL20-1 and 35S:AtAHL20-2 transgenic plants flowered later than the wild type, 35S:AtAHL20m1,35S:AtAHL20m2, ahl20-1 and ahl20-2 plants under LD conditions. e and f 35S:AtAHL20-1 and 35S:AtAHL20-2 transgenic plants flowered later than the wild type, 35S:AtAHL20m1, 35S:AtAHL20m2, ahl20-1 and ahl20-2 plants under SD conditions. When we overexpressed AtAHL20 protein carrying a point mutation in a conserved R-G-R core motif, the resultant transgenic plants flowered earlier than the wild type (c-f). Flowering time was calculated by counting the number days from sowing until the appearance of a 1 cm long primary bolt, as well as by counting the total number of primary rosette and cauline leaves present at bolting. The error bar denotes standard deviation (SD). Different letters indicate statistical significance (ANOVA; P < 0.05). n = at least 35 plants (between 35 and 48 plants per genotype). The experiment was repeated three times with similar outcomes