Fig. 1

AtAHL20 promoter-GUS expression pattern. Wild-type Arabidopsis plants were transformed with a construct encoding a pAtAHL20-GUS transcriptional fusion. Histochemical analysis was conducted on 6-day old seedlings grown on Murashige and Skoog (MS) agar plates under continuous white light, rosette leaves from 12-day old plants and floral structures of adult plants grown under LD conditions in the greenhouse. GUS histochemical staining patterns in (a), 6-day old pAtAHL20:GUS seedlings (b), rosette leaves from a 12-day old pAtAHL20:GUS plant showing expression in minor veins (c), pAtAHL20:GUS floral structure (d), close-up of pAtAHL20:GUS floral structure. GUS activity assays were performed three times with similar results. (e) Semi-quantitative PCR gel showing AtAHL20 tissue expression pattern in 7-day old seedling roots (R), 7-day old whole seedling (WS), adult flowering plant rosette leaf (L), 7-day old seedling hypocotyls (H), flower (F), siliques (S) and no reverse transcriptase negative control (NC). UBQ10 was used as a house keeping gene control