Fig. 6

Phylogenetic and expression analysis of the identified 97 GmPLCPs. a Phylogenetic analysis of the identified 97 GmPLCPs. Phylogenetic tree construction of GmPLCPs is based on the full-length deduced amino acid sequences using the programs RAxML, MEGA version 6.0 and MrBayes 3.2. The tree shows nine major phylogenetic classes (class 1 to class 9) indicated with different shadows. b Expression analysis of the identified 97 GmPLCPs in five RNS-related tissues in the Phytozome database. The expression values of GmPLCPs in the Phytozome database and the pheatmap packages in R were used to product the Heatmap. These tissues include Nodules (1), Root Hairs (2), Roots (3), Nodules. Symbiotic. Condition (4) and Roots. Symbiotic. Condition (5). c RNA-seq analysis of the identified 97 GmPLCPs in five different nodules of soybean. Our previous RNA-Seq data [18] and the pheatmap packages in R were used to product this Heatmap. These five nodule samples include the nodules in branching stage (6), flowering stage (7), fruiting stage (8), pod stage (9) and harvest stage (10), and the description of these five important developmental stages were shown in our previous research [18]