Fig. 5

PTI responses are enhanced in sl-MH-1 mutant. a ROS burst from MH86 and sl-MH-1 leaf discs treated with 400 nM chitin or 500 nM flg22 was detected at the indicated time points. Error bars represent the SE (n = 8). The data are from one of three independent experiments performed with similar results. B Callose deposition on MH86 and sl-MH-1 leaves after chitin treatment was imaged with a microscope under UV light (left). Number of the callose deposition per view was analysed by Image J (right). Bars represent mean values ± SE (n = 5). Statistically significant difference was indicated by ** (p < 0.01, Student’s t-test). This assay was performed in three independent replicates with similar results. c The relative transcriptional levels of KS4 in 3-week-old MH86 and sl-MH-1 leaves at the indicated time points after treated with 400 nM chitin. Rice ubiquitin-encoding gene (UBQ) was used as the internal control. Bars represent mean values ± SD (n = 3). Statistically significant difference was indicated by ** (p < 0.01, Student’s t-test). This assay was performed in two independent replicates with similar results