Table 3 Relative transcript abundance of RF-Oma1 measured by RT-qPCR using common indel characteristics of 200-kDa generative copies [n = 2 (ef1α of p2p2) or n = 3 (the others)]
s17 marker type (genotype) | Reference gene | Anther developmental stage | |
|---|---|---|---|
Meiosis | Tetrad | ||
p1p4 (NK-198 Rf1 heterozygous) | Actin | 0.43 ± 0.12 a | 0.39 ± 0.02 |
ef1α | 0.48 ± 0.14 | 0.48 ± 0.04 | |
p1p1 (NK-198 Rf1 homozygous) | Actin | 0.86 ± 0.21 | 0.76 ± 0.18 |
ef1α | 1.00 ± 0.16 | 1.05 ± 0.30 | |
p2p4 (NK-305 Rf1 heterozygous) | Actin | 0.16 ± 0.02 | 0.15 ± 0.01 |
ef1α | 0.20 ± 0.01 | 0.18 ± 0.06 | |
p2p2 (NK-305 Rf1 homozygous) | Actin | 0.28 ± 0.03 | 0.34 ± 0.01 |
ef1α | 0.30 ± 0.04 | 0.42 ± 0.03 | |
p4p4 (rf1rf1) | Actin | Not detected | Not detected |
ef1α | Not detected | Not detected | |