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Table 3 Relative transcript abundance of RF-Oma1 measured by RT-qPCR using common indel characteristics of 200-kDa generative copies [n = 2 (ef1α of p2p2) or n = 3 (the others)]

From: The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.)

s17 marker type (genotype) Reference gene Anther developmental stage
Meiosis Tetrad
p1p4 (NK-198 Rf1 heterozygous) Actin 0.43 ± 0.12 a 0.39 ± 0.02
ef1α 0.48 ± 0.14 0.48 ± 0.04
p1p1 (NK-198 Rf1 homozygous) Actin 0.86 ± 0.21 0.76 ± 0.18
ef1α 1.00 ± 0.16 1.05 ± 0.30
p2p4 (NK-305 Rf1 heterozygous) Actin 0.16 ± 0.02 0.15 ± 0.01
ef1α 0.20 ± 0.01 0.18 ± 0.06
p2p2 (NK-305 Rf1 homozygous) Actin 0.28 ± 0.03 0.34 ± 0.01
ef1α 0.30 ± 0.04 0.42 ± 0.03
p4p4 (rf1rf1) Actin Not detected Not detected
ef1α Not detected Not detected
  1. aMean ± SD