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Table 2 Relative transcript abundance of RF-Oma1 measured by RT-qPCR in male sterile plants with recessive rf1 alleles from different origins (n = 2)

From: The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.)

Line/ cross combination Total copy number of RF-Oma1 in zygote Reference gene Anther developmental stage
Meiosis Tetrad
TA-33BB-CMS 2 Actin 0.32 ± 0.08 a 0.17 ± 0.00
ef1α 0.37 ± 0.06 0.23 ± 0.01
TA-33BB-CMS x PI 615522 2 Actin 0.35 ± 0.01 0.21 ± 0.02
ef1α 0.35 ± 0.08 0.30 ± 0.05
TA-33BB-CMS x PI 518644 3 b Actin 0.59 ± 0.12 0.37 ± 0.03
ef1α 0.68 ± 0.08 0.55 ± 0.15
  1. aMean ± SD
  2. bExcluding orf20NK-219-1 because it is an apparent pseudogene