The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.)

Table 2 Relative transcript abundance of RF-Oma1 measured by RT-qPCR in male sterile plants with recessive rf1 alleles from different origins (n = 2)

Line/ cross combination	Total copy number of RF-Oma1 in zygote	Reference gene	Anther developmental stage
Line/ cross combination	Total copy number of RF-Oma1 in zygote	Reference gene	Meiosis	Tetrad
TA-33BB-CMS	2	Actin	0.32 ± 0.08 ^a	0.17 ± 0.00
TA-33BB-CMS	2	ef1α	0.37 ± 0.06	0.23 ± 0.01
TA-33BB-CMS x PI 615522	2	Actin	0.35 ± 0.01	0.21 ± 0.02
TA-33BB-CMS x PI 615522	2	ef1α	0.35 ± 0.08	0.30 ± 0.05
TA-33BB-CMS x PI 518644	3 ^b	Actin	0.59 ± 0.12	0.37 ± 0.03
TA-33BB-CMS x PI 518644	3 ^b	ef1α	0.68 ± 0.08	0.55 ± 0.15

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