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Fig. 4 | BMC Plant Biology

Fig. 4

From: The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.)

Fig. 4

Immunoblot analysis of total cellular proteins from immature anthers collected from TA-33BB-CMS (lane 1), NK-198 (lane 2), TA-33BB-CMS x PI 615522 (lane 3) and TA-33BB-CMS x PI 518644 (lane 4). Protein samples were electrophoresed in Blue Native polyacrylamide gels (4–16%). Size markers (kDa) are shown on the left. An arrow indicates the 200-kDa band that is the hallmark of post-translational interaction with preSATP6. Immunoblots were probed with anti-preSATP6 (a and b) or anti-COXI (c). Exposure times to X-ray film were 10 s and 1 min for a and b, respectively

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