Fig. 4
The expressional patterns of NnMADS genes in different tissues of N. nucifera. a The left panel shows the MIKCc sub-type members of type II MADS box genes; the upper panel of right side shows MIKC* sub-type members of type II MADS box genes, and the lower panel of the right side shows those of the type I. The FPKM values were collected from the online data as mentioned in M&M, and used for drawing the heatmap. b qRT-PCR analysis on the relative expressions of 14 representative NnMADS genes in different tissues of N. nucifera. Transcript level of each NnMADS were first normalized to those of the housekeeping gene NnActin and then compared to the expression level of each gene in leaf. Data are means ±SD (n = 3). L: Leaf; Pe: Petiole; three parts of the rhizome, such as Rt: rhizome tip, Rez: Rhizome elongation zone, Ri: Rhizome internode; R: Root; Fb: Flower bud; P: Petal; Being collected stamen, carpel, receptacle before pollination named as immature stamen (iSt), immature carpel (iC), immature receptacle (iRe); Being collected stamen, carpel, receptacle after pollination named as mature stamen (mSt), mature carpel (mC), mature receptacle (mRe); Sc: Seed coat; Co: Cotyledon