Fig. 5
From: Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice
Validation of RNA-seq data using real-time quantitative PCR for six potential AtPLC9 target genes. RNA-seq signals visualized using Integrated Genome Browser (IGB; http://www.bioviz.org) and real-time quantitative PCR quantification of the RNA levels for six candidate AtPLC9-regulated genes in wild-type (WT) and OE-7 plants before (CK) and after HS treatment. Values for RT-qPCR results are means ± SE (n = 3). Student’s t-test was used to calculate P values. ****P < 0.0001, ***P < 0.001, **P value < 0.01, statistics was analysed between OE-7-HS vs WT-HS