Fig. 3

Identification of genes regulated by AtPLC9 during response to heat stress in rice. a. Volcano plots of transcriptional changes generated from edgeR. Log2 of fold change is shown on the horizontal axis, and − log10 of the adjusted P value (FDR) is shown on the vertical axis. Red and blue dots represent significantly up- and downregulated genes, respectively. Green dots are genes with a nonsignificant change. Vertical and horizontal red dashed lines indicate the cutoff of log2-transformed fold change and -log10 of FDR, respectively. Numbers indicate the counts of genes showing up- and downregulation. b. Venn diagram showing the number of differentially expressed (left), upregulated (middle) and downregulated (right) genes in the four comparisons. WT and OE indicate the regulation of gene expression level between heat stress and control in WT and OE-7, respectively. CK and HS denote the change of expression values in OE-7 compared with WT under control and heat stress conditions, respectively