Fig. 2

Expression of AtPLC9 in rice improves acquired thermotolerance. a. 14-d-old rice (WT and transgenic lines OE-7, OE-9 and OE-10) grown under normal conditions (28 °C) were used as controls. White bar = 1 cm. b. 7-d-old rice (WT, OE-7, OE-9 and OE-10) grown under normal conditions (28 °C) were shifted to 37 °C for 30 min and then returned to normal conditions (28 °C) for 2 h before 45 °C HS treatment for 45 min. After the treatment, the seedlings were returned to 28 °C for another 7 d. White bar = 1 cm. c. The survival rate of WT, OE-7, OE-9 and OE-10 after HS. Each value is the mean ± SE, n = 3. d. Relative ion leakage quantification of plants after HS treatment. Each value is the mean ± SE, n = 3. e. Chlorophyll content of the seedlings after HS; 0.1 g of fresh tissue was used in each experiment. Each value is the mean ± SE, n = 3. Student’s t-test was used to calculate the P value. ** indicates P value < 0.01, * indicates P value < 0.05, statistics was analysed between OE-7/− 9/− 10 vs WT