Table 1 Frequency of simultaneous site-directed mutagenesis in the targeted loci Gly m Bd 28 K and Gly m Bd 30 K in T2 seeds
T1 plant numbera | Number of T2 seeds | ||||
|---|---|---|---|---|---|
Mutation | No mutation | Total | |||
Gly m Bd 28 K and Gly m Bd 30 K | Gly m Bd 28 K only | Gly m Bd 30 K only | |||
E1–1 | 18 | 11 | 8 | 2 | 39 |
E1–2 | 22 | 0 | 3 | 0 | 25 |
E1–5 | 9 | 4 | 3 | 1 | 17 |
E2–1 | 0 | 22 | 0 | 10 | 32 |
E2–2 | 1 | 20 | 0 | 5 | 26 |
K1–1 | 12 | 1 | 0 | 0 | 13 |
K1–3 | 16 | 3 | 4 | 2 | 25 |
K2–1 | 17 | 3 | 3 | 0 | 23 |
K2–2 | 20 | 7 | 4 | 0 | 31 |
K4–1 | 35 | 0 | 2 | 0 | 37 |
K5–1 | 50 | 0 | 0 | 0 | 50 |
K5–2 | 14 | 1 | 0 | 0 | 15 |
K6–1 | 12 | 3 | 0 | 0 | 15 |