Skip to main content

Table 1 Frequency of simultaneous site-directed mutagenesis in the targeted loci Gly m Bd 28 K and Gly m Bd 30 K in T2 seeds

From: Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis

T1 plant numbera Number of T2 seeds
Mutation No mutation Total
Gly m Bd 28 K and Gly m Bd 30 K Gly m Bd 28 K only Gly m Bd 30 K only
E1–1 18 11 8 2 39
E1–2 22 0 3 0 25
E1–5 9 4 3 1 17
E2–1 0 22 0 10 32
E2–2 1 20 0 5 26
K1–1 12 1 0 0 13
K1–3 16 3 4 2 25
K2–1 17 3 3 0 23
K2–2 20 7 4 0 31
K4–1 35 0 2 0 37
K5–1 50 0 0 0 50
K5–2 14 1 0 0 15
K6–1 12 3 0 0 15
  1. The presence of mutations was evaluated by CAPS analysis
  2. aThe letter and first number correspond to the number of the parental T0 plant