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Fig. 5 | BMC Plant Biology

Fig. 5

From: Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis

Fig. 5

Semi-quantitative RT-PCR of the Gly m Bd 28 K and the Gly m Bd 30 K genes mature seeds of representative double-mutant T3 and wild-type mature seeds. The expression level of targeted loci was evaluated based on the quantity amplified for 18S rRNA as a control endogenous gene. PCR was performed at 38 cycles for the Gly m Bd 28 K and at 30 cycles for the Gly m Bd 30 K and 18S rRNA

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