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Table 1 Ranking of the candidate reference genes, listed from most to least stable, in the samples from grapevine somatic embryo aggregates cultured in DM1 differentiation medium, as based on the geNorm, NormFinder and BestKeeper software programs

From: Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

Rank order

geNorm

NormFinder

BestKeepera

 

Gene name

M value

Gene name

Stability value

Gene name

Pearson correlation (r)

1

EF1-α(m)

0.346

EF1-α(m)

0.115

UBQ-L40

0.978

2

GAPDH(m)

0.346

GAPDH(m)

0.155

EF1-α(m)

0.963

3

AP47

0.411

AP47

0.212

GAPDH(m)

0.951

4

MDH(m)

0.465

TIP41

0.277

α-Tubulin

0.934

5

UBQ-L40

0.490

EF1-α

0.290

AP47

0.924

6

EF1-α

0.517

UBQ-L40

0.316

UBQ-10(m)

0.923

7

TIP41

0.539

MDH(m)

0.341

MDH(m)

0.911

8

α-Tubulin

0.566

UBQ-10(m)

0.374

EF1-α

0.905

9

UBQ-10(m)

0.588

SAND

0.389

TIP41

0.847

10

SAND

0.620

α-Tubulin

0.448

SAND

0.785

11

PP2A

0.654

PP2A

0.478

  

12

Actin

0.690

Actin

0.508

  

13

β-Tubulin

0.732

β-Tubulin

0.656

  

14

UBC

0.789

UBC

0.702

  

15

Cyclophilin

0.843

Cyclophilin

0.725

  
  1. aBestKeeper is able to analyze a maximum of ten genes; therefore, the five worst-ranked candidates in previous analyses by geNorm and NormFinder (PP2A, Actin, β-Tubulin, UBC and Cyclophilin) were excluded from the studied data sets