Fig. 7

Effect of exogenous ABA on the relative expression of the VvNCED1 gene in grapevine cv. Mencía somatic embryo aggregates cultured for 2 weeks over a semipermeable membrane on DM1 medium supplemented with 6% sucrose and then treated by pipetting directly onto the aggregates either a solution of 30% sucrose or a solution containing 30% sucrose and 10 μM ABA. The expression levels were calculated relative to the VvNCED1 gene expression in somatic embryo aggregates cultured under the same conditions but untreated with any solution (calibrator group). GAPDH (m) and EF1-α (m) were used as the reference genes for normalization. Data are presented as the means of two independent experiments with standard errors. The asterisk indicates a significant difference between the untreated samples and the treated samples (p < 0.05), as calculated using REST-2009© software