Fig. 3

Characterisation of candidate gene At4g16850. a Sequence variation in the 322 bp intergenic and 5’UTR region between VRN2 and At4g16850 transcription start site, and in the 5’UTR, between the large petal accession Dju-1 and small petal genotype Col-0. Two A/T insertions in Dju-1 relative to Col-0 are shown above the line, and insertions in Col-0 relative to Dju-1 are shown below the line. Additional File 5 shows a sequence alignment of these regions. b The coding regions of At4g16850 from the large petal accession Dju-1 and the small petal accession Col-0 were aligned to identify predicted protein sequence differences. The coding regions were analysed with InterPro to identify putative transmembrane and cytoplasmic protein domains, shown as coloured bars under the predicted coding sequence. Amino acid differences are shown as gray highlights. c Transient expression of At4g16850 coding region fused to GFP at its C-terminus from the 35S promoter in Col-0 petal protoplasts. A known plasma-membrane protein TMK4 fused to RFP was used to reveal co-location in the plasma membrane. The white colour in the overlay reveals co-location of At4g16850-GFP and TMK4-RFP