Fig. 5

Detection of expression and putrescine contents in transgenic and RNAi hairy roots. a The phenotype of the hair root (GFP, control; BrrADC2.2-OE, overexpression of BrrADC2.2 in hair root; BrrICE1.1-OE, overexpression of BrrICE1.1 in hair root). b Laser confocal detection of overexpressed GFP, BrrADC2.2-OE and BrrICE1.1-OE in the hairy roots. CN: cell nucleus, CM: cell membrane. Bar = 20 μm. c d The expression levels of BrrADC2.2 and putrescine contents in BrrADC2.2-OE transgenic and BrrADC2.2-RNAi hairy roots compared with those of the CK. e Laser confocal detection of overexpressed BrrICE1.1 and its amino acid sequence mutant (BrrICE1.1-m) protein localization in N. benthamiana leaves. Bar = 20 μm. f The structure of the AtICE1 and BrrICE1.1 protein at the N-terminus (1–30). The red line represents a single peptide in the BrrICE1.1 protein. g The expression levels of BrrICE1.1 and BrrADC2.2 in BrrICE1.1-OE transgenic hair roots (left) and BrrICE1.1 and BrrADC2.2 in BrrICE1.1-RNAi transgenic hair roots (right). h Putrescine contents in BrrICE1.1-OE transgenic and BrrICE1.1-RNAi hairy roots. In c, d, g and h, the data are the mean of three replicates ± SD, and the asterisks indicate significant differences compared with the CK (*P < 0.05, **P < 0.01, Student’s t test)