Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: BrrICE1.1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of turnip (Brassica rapa var. rapa)

Fig. 4

Binding motif analysis in the target regions of turnip BrrICE1.1 and interaction analysis of BrrICE1.1 with the promoter of the differentially expressed genes in the polyamine pathway in vivo and in vitro. a The potential MYC-binding site (CANNTG) of BrrICE1.1. The binding sequences of the BrrICE1.1 with BrrAIH1.1, BrrAIH1.2, BrrADC2.1, and BrrADC2.2 are shown in red box. b Yeast one-hybrid assays showed that the MYC element mediates BrrICE1.1 binding to the BrrAIH1.1, BrrAIH1.2, BrrADC2.1, and BrrADC2.2 promoters, and the BrrAIH1.1, BrrAIH1.2, BrrADC2.1, and BrrADC2.2 promoters were mutated (deleted MYC element) to abolish the MYC element alone. The experiments were repeated three times with the same results. c BrrICE1.1 activated the activity of BrrAIH1.1, BrrAIH1.2, BrrADC2.1, and BrrADC2.2 in vivo. N. benthamiana leaves. Representative images of N. benthamiana leaves 72 h after infiltration are shown. d ChIP experiment using BrrICE1.1-6flag transgenic hair root. The structure of the BrrADC2.2 gene promoter. The primer sequence regions used for ChIP assays are marked with a horizontal line to the left of the TSS. The control primer sequence (GD) was on the left side of TSS. ChIP-qPCR showing binding of BrrICE1.1 to BrrADC2.2 promoters in vivo. WT and BrrADC2.2-GD were used as negative controls. The data are the mean of three replicates ± SD, and the asterisks indicate significant differences compared with IgG (*P < 0.05, **P < 0.01, Student’s t test)

Back to article page