Genome-wide characterization and expression analysis of the heat shock transcription factor family in pumpkin (Cucurbita moschata)

Table 3 KaKs calculation and estimated divergence time for the duplicated CmHsf gene pairs

Duplicated CmHsf gene pairs	Identity (%)	E-value	Gene alignment coverage	Ka	Ks	Ka/Ks	Divergence time (MYA)
CmHsf12-CmHsf10	95.12	1.00E-12	0.975	0.832	1.972	0.422	65.742
CmHsf26-CmHsf9	95.12	1.00E-12	0.910	0.126	0.462	0.273	15.416
CmHsf22-CmHsf27	85.09	0	0.893	0.145	0.673	0.215	22.432
CmHsf13-CmHsf28	85.09	0	0.946	0.232	0.436	0.531	14.535
CmHsf30-CmHsf16	86.13	0	0.931	0.074	0.305	0.242	10.168
CmHsf6-CmHsf19	86.13	0	0.863	0.080	0.336	0.238	11.204
CmHsf21-CmHsf1	87.83	0	0.812	0.066	0.542	0.121	18.083
CmHsf3-CmHsf31	87.83	0	0.814	0.106	0.431	0.246	14.354
CmHsf32-CmHsf35	87.97	0	0.981	0.181	0.542	0.335	18.053

Note: We used the KaKs calculator to calculate the Ka/Ks. Ks, synonymous substitutions; Ka, nonsynonymous substitutions

ISSN: 1471-2229