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Table 2 Stability of expression of the 20 candidate reference genes, as calculated by BestKeeper

From: Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis

Ranking

H. robusta

-leaves

H. robusta

-young stems

H. robusta

-leaves & young stems

Different tissues

4 °C treatment

MeJA treatment

PEG6000 treatment

All samples

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

gene

CV ± SD

1

UBC17

1.16 ± 0.23

HIS1

1.40 ± 0.31

UBC17

1.73 ± 0.34

HIS6

5.41 ± 1.38

18S

0.73 ± 0.16

60S-18

0.67 ± 0.14

PPIA26

3.89 ± 0.87

MUB1

3.95 ± 0.87

2

18S

1.62 ± 0.36

UBC17

1.70 ± 0.33

18S

2.12 ± 0.47

MUB1

5.48 ± 1.16

60S-18

0.83 ± 0.18

EF1

0.83 ± 0.14

60S-18

4.48 ± 1.02

PPIA95

4.16 ± 0.96

3

HIS6

2.05 ± 0.48

TUB-β

1.84 ± 0.44

MUB1

2.54 ± 0.56

PPIA95

6.24 ± 1.36

PPIA95

0.90 ± 0.21

HIS1

0.85 ± 0.19

PPIA95

5.10 ± 1.24

18S

4.58 ± 1.02

4

UBC5B

2.06 ± 0.37

18S

1.87 ± 0.41

HIS6

2.69 ± 0.62

SAMDC

6.42 ± 1.34

EF1

1.13 ± 0.21

TUB-β

0.87 ± 0.20

ACT7

5.18 ± 1.18

PGK

4.90 ± 0.99

5

PP2C59

2.31 ± 0.45

PGK

2.05 ± 0.40

HIS1

3.09 ± 0.70

60S-18

6.57 ± 1.34

PGK

1.19 ± 0.24

PPIA95

0.88 ± 0.20

HIS6

5.26 ± 1.42

PP2C59

5.13 ± 1.02

6

SAMDC

2.84 ± 0.63

ACT7

2.25 ± 0.44

UBC5B

3.09 ± 0.54

UBC5B

7.32 ± 1.30

TUB-α

1.32 ± 0.27

TIP41

0.89 ± 0.20

TUB-β

5.32 ± 1.36

TUB-α

5.14 ± 1.09

7

MUB1

2.95 ± 0.65

MUB1

2.26 ± 0.50

PPIA95

3.17 ± 0.74

EF2

7.47 ± 1.48

ACT7

1.51 ± 0.33

UBC17

0.97 ± 0.19

PGK

5.43 ± 1.17

TIP41

5.28 ± 1.17

8

HIS1

3.06 ± 0.71

TIP41

2.46 ± 0.52

PP2C59

3.31 ± 0.66

HIS1

7.54 ± 1.77

SAMDC

1.58 ± 0.34

60S-13

0.97 ± 0.21

SAMDC

5.63 ± 1.32

SAMDC

5.33 ± 1.15

9

ACT7

3.12 ± 0.65

PPIA95

2.75 ± 0.64

TUB-α

3.42 ± 0.72

PGK

27.62 ± 1.51

EF2

1.65 ± 0.35

SAMDC

1.34 ± 0.28

PP2C57

5.76 ± 1.24

UBC5B

5.36 ± 0.97

10

TUB-α

3.17 ± 0.68

UBC5B

2.81 ± 0.48

ACT7

3.66 ± 0.74

18S

7.75 ± 1.66

TUB-β

1.67 ± 0.43

HIS6

1.49 ± 0.34

18S

6.07 ± 1.44

ACT7

5.37 ± 1.14

11

TIP41

3.46 ± 0.78

HIS6

2.87 ± 0.65

SAMDC

3.85 ± 0.84

PP2C59

7.95 ± 1.59

HIS6

1.92 ± 0.44

18S

1.57 ± 0.34

MUB1

6.34 ± 1.43

UBC17

5.48 ± 1.11

12

PPIA95

3.61 ± 0.84

60S-13

3.05 ± 0.62

PGK

3.96 ± 0.80

ACT7

8.07 ± 1.68

60S-13

2.09 ± 0.47

EF2

1.64 ± 0.32

UBC5B

6.37 ± 1.27

60S-18

5.56 ± 1.19

13

PGK

4.16 ± 0.86

TUB-α

3.36 ± 0.70

TUB-β

3.98 ± 0.93

TIP41

8.08 ± 1.77

MUB1

2.11 ± 0.47

ACT7

1.68 ± 0.35

EF2

6.42 ± 1.43

HIS1

5.86 ± 1.37

14

PP2C57

4.35 ± 0.88

EF2

3.52 ± 0.65

TIP41

4.34 ± 0.94

TUB-α

8.09 ± 1.70

PP2C57

2.21 ± 0.44

PP2C57

1.84 ± 0.38

EF1

6.61 ± 1.32

TUB-β

6.09 ± 1.47

15

60S-13

4.45 ± 0.97

PP2C59

3.99 ± 0.81

60S-18

4.79 ± 1.00

60S-13

8.83 ± 2.03

UBC5B

2.23 ± 0.41

UBC5B

1.93 ± 0.35

UBC17

7.03 ± 1.55

HIS6

6.61 ± 1.59

16

TUB-β

4.75 ± 1.08

EF1

4.21 ± 0.69

60S-13

5.07 ± 1.07

UBC17

8.97 ± 1.81

UBC17

2.31 ± 0.47

PGK

2.00 ± 0.40

PP2C59

7.04 ± 1.48

EF2

6.82 ± 1.38

17

EF2

4.83 ± 0.97

60S-18

4.48 ± 0.91

EF1

5.21 ± 0.87

TUB-β

9.33 ± 2.19

PP2C59

2.51 ± 0.48

TUB-α

2.00 ± 0.42

TUB-α

7.13 ± 1.62

60S-13

7.61 ± 1.70

18

60S-18

4.84 ± 1.04

SAMDC

4.85 ± 1.04

EF2

5.38 ± 1.04

EF1

9.44 ± 1.57

HIS1

2.52 ± 0.59

MUB1

2.28 ± 0.49

TIP41

7.51 ± 1.75

PP2C57

7.77 ± 1.66

19

EF1

6.03 ± 1.04

PP2C57

5.49 ± 1.30

PP2C57

8.84 ± 1.94

PP2C57

12.30 ± 2.71

TIP41

3.11 ± 0.71

PP2C59

3.27 ± 0.64

HIS1

7.70 ± 1.97

EF1

8.11 ± 1.43

20

PPIA26

8.36 ± 2.02

PPIA26

5.99 ± 1.67

PPIA26

10.94 ± 2.84

PPIA26

15.21 ± 3.73

PPIA26

4.58 ± 1.03

PPIA26

4.46 ± 1.04

60S-13

10.75 ± 2.70

PPIA26

9.83 ± 2.37