Table 1 Candidate reference genes, primer sequences, and characteristics of PCR amplification in T. ciliata
Gene symbol | Gene Name | Primer: Forward/reverse | Amplification product size (bp) | standard curve | En | R2 |
|---|---|---|---|---|---|---|
ACT7 | Actin-7 | F: TGATTGGGATGGAAGCAGCA R: GAACATGGTTGAACCGCCAC | 122 | y = − 3.5133x + 29.711 | 0.9259 | 0.9931 |
PGK | Phosphoglycerate kinase | F: CCGCAAGCTTCTTTGCGATT R: GGCTTGGATATTGGACCCGA | 145 | y = − 3.2649x + 26.93 | 1.0244 | 0.9985 |
60S-18 | 60S ribosomal protein L18a-1 | F: GCCTGGATGCCTTGTATGTTG R: GGGAAAGCACCAAGCAGTTTC | 108 | y = −3.5672x + 27.862 | 0.9332 | 0.9993 |
60S-13 | 60S ribosomal protein L13–1 | F: CCAACATGGCACTCATTCGC R: TTCCCAAGATGTGCTCGCAA | 200 | y = −3.4076x + 29.173 | 0.9654 | 0.9929 |
HIS6 | Histone deacetylase 6 | F: ATTGTCCGGTGATAGGTTGGG R: GTCTCGTAGCACCAACAACG | 153 | y = −3.4932x + 29.484 | 0.9332 | 0.9965 |
HIS1 | Histone acetyltransferase MCC1 | F: CTGCACGAATTGTGCTGGTC R: ACTGCACGACATGTTGGGAT | 193 | y = −3.5229x + 30.411 | 0.9225 | 0.9959 |
PP2C57 | Protein phosphatase 2C 57 | F: TGTTGCAGCTTTACAAGGCG R: TGAACAAATCACCGCCTCCA | 185 | y = −3.3057x + 32.193 | 1.0068 | 0.9949 |
PP2C59 | Probable protein phosphatase 2C 59 | F: TAAGCGATCGCCAACAAGGA R: CACGAGCTGCTGAGTATGTGA | 194 | y = −3.3119x + 27.157 | 1.0042 | 0.9974 |
UBC5B | Ubiquitin-conjugating enzyme E2 5B | F: GGAGGACCCATGATTGTTGC R: TCGAAGCGGATCTTGAAGGAG | 116 | y = −3.3156x + 25.529 | 1.0026 | 0.9987 |
UBC17 | Ubiquitin-conjugating enzyme E2–17 kDa | F: GCGTCGAAACGCATCTTGAA R: GAAACACCCCTCCCGCATAA | 148 | y = −3.4489x + 26.966 | 0.9496 | 0.999 |
EF1 | elongation factor 1-alpha-like | F: CCGACCTTCTTCAGGTAGGAA R: TCCAAGGATGGTCAGACTCG | 164 | y = −3.4295x + 23.45 | 0.9570 | 0.9915 |
EF2 | elongation factor 1-alpha-like | F: CACCCTTGGTGTGAAGCAAA R: GGTTGGTGGACCTCTCAATCA | 200 | y = −3.4128x + 27.077 | 0.9561 | 0.9992 |
PPIA26 | Peptidyl-prolyl cis-trans isomerase CYP26–2 | F: GAAGCTGAAGTTGGTTGCCC R: GACGACCAGGGCTGAAACAT | 147 | y = −3.4393x + 30.33 | 0.9532 | 0.9952 |
PPIA95 | Peptidyl-prolyl cis-trans isomerase CYP95 | F: ACCCGGCCTCTTATCTATGC R: ACAAGCTCCCCGAATACCAC | 117 | y = −3.4295x + 23.45 | 0.9041 | 0.9915 |
SAMDC | S-adenosylmethionine decarboxylase proenzyme | F: AGCGATCTGCTATGACCCTG R: CCCGCAGAACCTGATTGGTC | 102 | y = −3.3179x + 29.683 | 1.0017 | 0.9994 |
18S | 18S rRNA factor 2 | F: GCTGCTAAGAGAGAGCGGG R: GGGAGCTCAGAATGGGTTCG | 128 | y = −3.4317x + 30.095 | 0.9561 | 0.9987 |
TUB-α | Tubulin alpha-3 chain | F: TACAACAGTTGGCGGCTGAT R: TGTACCGCGGAGATGTTGTT | 137 | y = −3.3568x + 29.206 | 0.9857 | 0.9998 |
TUB-β | Tubulin beta-5 chain | F: ACACACGCTGGACTTGACAT R: TCGCTACCTAACTGCTTCGG | 139 | y = −3.3349x + 32.62 | 0.9946 | 0.9996 |
MUB1 | Membrane-anchored ubiquitin-fold protein 1 | F: GCATTCTTGCTCAATGGCCT R: GGTTGTAACTCCACCAGGGA | 152 | y = −3.3956x + 28.572 | 0.9701 | 0.9984 |
TIP41 | TIP41-like protein | F: TGGTTGGAAGCAGGAAGGTT R: TTCACTTCCGCAGTATGGTG | 133 | y = −3.3332x + 31.127 | 0.9953 | 0.9992 |
MYB3 | Transcription factor MYB3 | F: CGCACCCATAACAACTCCCA R: TCTTTCACTTACTCCCTCTTCAGC | 178 | y = −3.4246x + 32.543 | 0.9589 | 0.9968 |