Table 2 Primers used in guide-RNA synthesis
Primers | Sequence (bold: guide-RNA sequences) |
|---|---|
sgRNA1-F | GAAATTAATACGACTCACTATAGCATCTTCATCTGCCTTACAGTTTTAGAGCTAGAAATAGCAAG |
sgRNA2-F | GAAATTAATACGACTCACTATAGGATGACCCTTGTTTACAAAGTTTTAGAGCTAGAAATAGCAAG |
sgRNA-R | AAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
crRNA1-F | AAGGTGATGCATAATTTGTTCAAATCTACACTTAGTAGAAATT |
crRNA2-F | CCAGTTTCTAACTTATGTGTCCCATCTACACTTAGTAGAAATT |
crRNA-R | GAAATTAATACGACTCACTATAGGGAATTTCTACTAAGTGTAGAT |