Fig. 5

CM334 callus protoplasts for CRISPR/LbCpf1 RNP-mediated CaMLO2 editing. a Indel frequency of LbCpf1-crRNA1 and LbCpf1-crRNA2 transfected into CM334 callus protoplasts at 48 h post-incubation. Vertical bars represent the mean ± standard deviation (n = 3). The asterisk indicates significant difference as P < 0.01 compared with LbCpf1 only based on ANOVA. b Indel patterns of LbCpf1-crRNA1 and LbCpf1-crRNA2 editing of CaMLO2 loci. Total reads were obtained by targeted deep sequencing. Indel frequency (%) was calculated as the number of measured reads divided by the number of total reads. Red, PAM sequences of TTTN; Blue, CRISPR target sequence; Red dash lines and -, deleted nucleotides