Fig. 3From: A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuumDempsey leaf protoplasts for CRISPR/Cas9 RNP-mediated CaMLO2 editing. a Dempsey leaf protoplast expressed nucleus-targeted GFP:NLS. b Indel frequency of Cas9-sgRNA1 and Cas9-sgRNA2 transfected into Dempsey leaf protoplasts. Vertical bars represent the mean ± standard deviation (n = 3). Asterisk indicates significant different at P < 0.01 compared with Cas9-only based on ANOVA. c Indel patterns of Cas9-sgRNA1 and Cas9-sgRNA2 editing of CaMLO2 loci. Total reads were obtained by targeted deep sequencing. Indel frequency (%) was calculated as the number of measured reads divided by the number of total reads. Red, PAM sequences; Blue, CRISPR target sequence; Red dash lines and -, deleted nucleotides; Red letters and +, inserted nucleotidesBack to article page