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Fig. 2 | BMC Plant Biology

Fig. 2

From: A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuum

Fig. 2

In vitro cleavage assay for CRISPR/Cas9 or CRISPR/Cpf1 RNP-mediated CaMLO2 gene in two peppers. a Target locus of CaMLO2 gene, four designed guide RNAs (sgRNA1 and sgRNA2 for Cas9 and crRNA1 and crRNA2 for LbCpf1) and a specific primer pair, F and R. b Target sequences of the four guide RNAs. c In vitro cleavage assay with preassembled Cas9-only as a control, Cas9-sgRNA1 and Cas9-sgRNA2 for CaMLO2 gene of CM334 or Dempsey. d In vitro cleavage assay with preassembled LbCpf1-only as a control, LbCpf1-crRNA1 and LbCpf1-crRNA2 for CaMLO2 gene of CM334 and Dempsey

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