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Fig. 1 | BMC Plant Biology

Fig. 1

From: A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuum

Fig. 1

Schematic overview of clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome editing in pepper protoplasts. Preassembled CRISPR-RNP complexes (for example, CRISPR/Cas9 RNP) were delivered to purely isolated protoplasts from the leaf or callus of CM334 and Dempsey peppers by PEG-mediated transfection. The delivered CRISPRs RNP was targeted to the nucleus of the pepper protoplasts and subsequently cleaved the target locus. Scale bars, 20 μm

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BMC Plant Biology

ISSN: 1471-2229

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