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Fig. 3 | BMC Plant Biology

Fig. 3

From: Genome-wide transcriptional changes triggered by water deficit on a drought-tolerant common bean cultivar

Fig. 3

Validation of selected DEGs determined by semi-quantitative RT-PCR. a. RT-PCR analysis by agarose gel electrophoresis of up- (PYL4, XTH6, CESA4, and CSLD5) and down-regulated (HSP70, HSFA2, FTSH6, and HYH) genes are shown for PS. Constitutive genes from our RNA-Seq data (EIF5A) and previously reported (SKIP16) were used in the analysis. Representative gels corresponding to 32 (CESA4, CSLD5, and HSP70) and 34 (PYL4, XTH6, HSFA2, FTSH6, HYH, EIF5A, and SKIP16) cycles are shown. (C, Control; D, Drought). b. Density analysis of PCR bands was determined by ImageJ software and normalized using the EIF5A constitutive internal control corresponding to each condition (a.u. - arbitrary units). Graphical representation of mean ± SE of at least three independent replicates. One-way ANOVA was used to compare the statistical difference between measurements (P < 0.05). Samples tested for the same gene are indicated by lowercase letters. Significant differences compared to the control samples are indicated by different numbers

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