Fig. 1

Tissue-expression profiles, subcellular localization and targeted disruption of PpATG3 gene. a PpATG3 expression profiles in different P. patens tissues. The expression data was retrieved from a previous research by Ortiz-Ramírez et al. b Subcellular localization of PpATG3. Confocal microscopy images of P. patens protoplasts by PEG-mediated transformation with empty vector (EV) or with p35S:PpATG3-eGFP construct. The scale bar = 10 μm. c Targeted disruption of PpATG3 gene and PCR confirmation. Schematic representation showing deletion of Exons 4–5 that corresponds to removal of a 573 bp genomic region and insertion of a 2078 bp nptII cassette. Right and left arrows were indicated forward and reverse primers, respectively. PCR analysis was used to verify genomic insertion of nptII cassette and loss of PpATG3 transcripts. Primer pairs of P5/C1 and C2/P6 were used for verifying double-ended insertion of the nptII cassette at genomic level. Primer pairs of P7/P8 and C3/C4 were used for verifying the loss of PpATG3 transcripts and the expression of nptII cassette, respectively. PpUbiquitin and PpAdePRT were used as a DNA or cDNA template quality control, respectively. The fragment length and DNA size markers were shown on the gel right and left, respectively