Fig. 8

TaWRKY42-B directly binds to the promotor of AtLOX3 and TaLOX3, affecting transcription of TaLOX3. a Diagram of probes against the AtLOX3 promoter region for EMSA. b Interaction between TaWRKY42-B and the promoter region of AtLOX3 analyzed by EMSA. c Diagram of probes against the TaLOX3 promoter region for EMSA. d Interaction between TaWRKY42-B and the promoter region of TaLOX3 analyzed by EMSA. TaWRKY42-B-MBP fusion protein mixed with labeled probes, with 100× or 200× unlabeled probes serving as competitors. The presence (+) or absence (−) of specific probes. Numbers above the top of the bands indicate the relative binding strength of the TaWRKY42-B-MBP fusion protein and labeled probes after normalization to the control group. e Diagrams of constructs in the transient expression assay. f TaWRKY42-B activated the expression of TaLOX3 in dual-luciferase reporter system in wheat protoplasts. Renilla luciferase was used for normalization. 35S:GFP act as a negative control. g Measurement of JA content in the 7th and 8th rosette leaves of three- and five-week-old Col-0 and TaWRKY42-B-OE lines, respectively. (Error bars indicate SD. Asterisks indicate significant differences in SPAD and ion leakage between the negative control and TaWRKY42-B group. Student’s t-test, *P < 0.05, **P < 0.01)