Fig. 3

Tissue and subcellular expression pattern of TaWRKY42-B in wheat. a-b Tissue expression pattern of TaWRKY42-B was examined in different tissues (Flag leaf, Spike, 1st mature leaf from the top, Mature leaf 1; 2nd mature leaf from the top, Mature leaf 2; Stem, Root, and Seed) of 6-month-old wheat plants by qRT-PCR. c Subcellular localization of TaWRKY42-B-GFP fusion protein in the wheat protoplast. The control experiment employed a simple GFP protein. Bars = 10 μm. All experiments were repeated at least three times. d Diagrams of constructs in the transcriptional activity assay. e Measurement of relative LUC reporter activity after transient expression of vectors as shown in (d). (Error bars indicate SD. Asterisks indicate significant differences. Student’s t-test, *P < 0.05, **P < 0.01)