Fig. 2

Spatiotemporal expression pattern of TaWRKY42-B in wheat. a Expression pattern of TaWRKY42-B was detected at four different developmental stages of wheat flag leaves (Y, M, ES, and LS). Chlorophyll content (b) and ion leakage rate (c) of leaves as shown in (a). d-f Transcription level of senescence-associated genes TaWRKY42-B, TaSAG3 and TaSAG5 in leaves as shown in (a). g-i Expression level of TaWRKY42-B and TaSAG3 in tip, middle and base of a senescent wheat flag leaf. (Error bars indicate standard deviation (SD). Asterisks indicate significant differences. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001)