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Fig. 6 | BMC Plant Biology

Fig. 6

From: Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

Fig. 6

Protein-protein interaction (PPI) networks of the SDEPs and qRT-PCR analysis of gene expression levels. a PPI networks of the SDEPs were analyzed using the STRING database (v. 11.0) and visualized using the Cytoscape software (v. 3.6.1). The color bar represents gradients of Log2 transformed HTS/Cont ratios of the SDEPs. Red balls represent up-regulated SDEPs and blue balls represent down-regulated SDEPs. Black broken cycles represent the two enriched PPI clusters. b qRT-PCR analysis of gene expression levels in leaves of two-week-old maca seedlings exposed to HTS (42 °C) for 12 h. Two-week-old maca seedlings grown at 25 °C for 12 h were used as controls. Nineteen SDEPs implicated in the ‘protein processing in endoplasmic reticulum’ pathway were selected and subjected to qRT-PCR analysis using the same samples as for TMT proteomic data. LmACT2 was used as an internal reference. Results are presented as means ± SD of three biological replicates and two technical replicates (n = 6). c Protein ratios of 19 SDEPs implicated in the ‘protein processing in endoplasmic reticulum’ pathway. Results are presented as means ± SD of the three proteomic data

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