Table 1 Summary of Selaginella assemblies
S. sellowii | S. lepidophylla | S. denticulata | ||||
Total Raw Reads | 138,288,472 | 199,259,295 | 114,231,403 | |||
Total Trimmed Reads | 125,956,200 | 182,787,767 | 104,700,744 | |||
Trinity | SOAPdenovo | Trinity | SOAPdenovo | Trinity | SOAPdenovo | |
rnaQUAST | ||||||
Contigs | 56,287 | 48,843 | 64,189 | 66,262 | 62,111 | 60,458 |
Contigs > 1000 bp | 37,086 | 10,561 | 31,815 | 10,277 | 32,281 | 14,454 |
Longest contig (bp) | 27,152 | 39,804 | 22,517 | 38,874 | 20,697 | 21,926 |
Average length (bp) | 2631.69 | 953.46 | 1962.86 | 780.02 | 1650.37 | 798.22 |
N50 length | 4482 | 4100 | 4061 | 4057 | 2825 | 2397 |
Total Assembled Bases | 148,130,112 | 46,570,041 | 125,993,766 | 51,685,348 | 102,506,311 | 48,258,718 |
GC content (%) | 53.22% | 52.92% | 50.84% | 50.93% | 49.74% | 49.54% |
Predicted genes (GeneMarkS-T) | 27,005 | 8,189 | 29,728 | 10,146 | 32,874 | 14,626 |
BUSCO | ||||||
Complete | 95.10% | 70.60% | 93.10% | 69.00% | 98.30% | 88.10% |
Bowtie2 | ||||||
Overall Alignment Rate | 96.17% | 87.38% | 95.08% | 82.89% | 96.99% | 88.48% |
DETONATE | ||||||
RSEM-EVAL score | −6.50 × 109 | −8.20 × 109 | −10.4 × 109 | −14.3 × 109 | −5.34 × 109 | − 6.95 × 109 |