Table 3 Characteristics of 12 compound microsatellite loci developed for A. venetum across all the samples. Shown for each locus are the locus name, the forward (F) and reverse (R) primer sequence, allele size, repeat motif, genetic characteristics and GenBank accession number
Locus | Forward | Reverse | Size (bp) | SSR motif | NA | HO | HE | PIC | HWE | GenBank accession number |
|---|---|---|---|---|---|---|---|---|---|---|
Av02 | AAAAATGGGCAATGGTGGGC | AGGCGTAGGTGAAGAGGAGT | 224 | (AT)11 | 5 | 0.515 | 0.511 | 0.462 | NS | MT737291 |
Av08 | AATCAGCCACCGAGTTACCG | ACCTCCTGCAAGCTGAATCC | 220 | (CAT)6 | 5 | 0.653 | 0.639 | 0.574 | NS | MT737292 |
Av13 | ACGAGAAGTTGGAAACAGACCA | GTATTTGGTGTCTTCGGCGC | 269 | (GT)6 | 3 | 0.050 | 0.049 | 0.048 | ND | MT737293 |
Av15 | ACTCGTTGGACATGATGTGCT | GGACCTTCTCATCAGCCTCG | 208 | (CT)8 | 3 | 0.228 | 0.204 | 0.184 | ND | MT737294 |
Av21 | AGCAGGGGAGAAGAATGCAC | GGGTCTTGATGAGGTGAGGG | 250 | (ACAA)6 | 4 | 0.109 | 0.114 | 0.110 | ND | MT737295 |
Av33 | CCAAACCACACAGCTCAACG | CCAAACCACACAGCTCAACG | 180 | (AT)9 | 3 | 0.337 | 0.339 | 0.312 | ND | MT737296 |
Av39 | CGCTTGCTGCCTCATCATTC | CCCTCTCACACCATCCCAAC | 271 | (TGC)6 | 3 | 0.545 | 0.651 | 0.575 | NS | MT737297 |
Av43 | CTGCATTCCCGCAAGTAACAG | TGATGCAGCTTAGGAGGGTC | 258 | (AAG)5 | 2 | 0.356 | 0.294 | 0.250 | ND | MT737298 |
Av55 | GCTCCGAGAAATCCTGCTCA | GCACTGCACCCTCCTACTAC | 218 | (AG)9 | 3 | 0.030 | 0.030 | 0.029 | ND | MT737299 |
Av63 | GGGTTTTGCTTCTGGGCATG | GAGCCAATCCGAACCCCAC | 157 | (TC)9 | 2 | 0.327 | 0.301 | 0.254 | ND | MT737300 |
Av75 | TCACTAGTACCCACCACCCC | AGTGGTGGCGTTGCTATGAA | 212 | (GT)6(AT)6 | 2 | 0.485 | 0.490 | 0.369 | NS | MT737301 |
Av88 | TGCATCATGTAGGGTACACACC | GCAAGTGTTCGCTGAGTTCC | 159 | (AAT)7 | 4 | 0.396 | 0.487 | 0.400 | NS | MT737302 |
Mean | Â | Â | Â | Â | 3.25 | 0.336 | 0.342 | 0.297 | Â | Â |