Fig. 9
From: Integrated sRNAome and RNA-Seq analysis reveals miRNA effects on betalain biosynthesis in pitaya
RLM-5′-RACE validation of miRNA-mediated cleavage of target genes. (A1-A7) ‘Guanhuabai’ pitaya. (B1-B7) ‘Guanhuahong’ pitaya. Top strand (black) depicts a miRNA complementary site, and bottom strand (red) depicts a miRNA complementary site the miRNA. Watson–Crick pairing (:) are indicated. Blue arrows indicate the cleavage sites of targets and the numbers show the frequency of the clones sequenced. Red arrows indicate the cleavage site of miRNA on target gene occurring at the 10th nucleotide from the 5′-end of miRNA in the binding region and the numbers show the frequency of the clones sequenced. The cleavage sites outside of the displayed sequence are not shown