Fig. 2From: Heterotrimeric G-protein α subunit (LeGPA1) confers cold stress tolerance to processing tomato plants (Lycopersicon esculentum Mill)LeGPA1 localization within cells. Arabidopsis mesophyll protoplasts were co-transformed with p35S-LeGPA1-GFP and a plasma membrane marker PM-rk. We co-transformed Arabidopsis mesophyll protoplasts using p35S-LeGPA1-GFP and the PM-rk plasma membrane marker. a The p35S-LeGPA1-GFP plasmid. b Arabidopsis mesophyll protoplasts following LeGPA1-GFP fusion protein expression. c Arabidopsis mesophyll protoplasts following Pm-rk expression. d Chloroplasts within Arabidopsis protoplasts. e A bright-field image of Arabidopsis mesophyll protoplasts. f Merged fluorescent images collected from LeGPA1-GFP and PM-rk expressing protoplasts. Scale bars = 7.5 μmBack to article page