Fig. 6

Validation of CRISPR/Cas9-induced mutations at both AhNFR5A and AhNFR5B DNA sequence sites. F1 ~ F10: alignment of genomic sequences cloned from the PCR products using DNA samples F1 ~ F10 of transgenic AhNFR5 hairy roots as templates. NFR5AB1 and NFR5AB2 represent two targets. Wild-type sequences are in black, deletions are shown as dots, and insertions are shown in blue. ‘-’, ‘+’ and ‘0’ on the right represent nucleotide deletions, insertions and no mutation, respectively