Fig. 4

Validation of CRISPR/Cas9-induced long fragment deletions at both AhNFR1A2 and AhNFR1B2 DNA sequence sites. H1-S, H2-S, H5-S, H7-S, and H10-S: alignment of genomic sequences cloned from the truncated PCR products using DNA samples H1, H2, H5, H7, H10 of transgenic AhNFR1 hairy roots as templates. Target 1 and target 2 represent target NFR1AB1 and target NFR1AB2. Wild-type sequences are in black, deletions are shown as dots, and insertions are shown in blue. ‘-’ on the right represent nucleotide deletions. PAM, protospacer adjacent motif