Fig. 6

Identification of the cotton fiber quality trait-associated candidate gene Gh_D06G1908 on Dt06. a Manhattan plots for FS and FL on Dt06. The dashed line indicates the significance threshold (P < 10^− 3). b Local Manhattan plot and LD heatmap of the genomic region 56.1–64.2 Mb on Dt06. The red dashed line indicates the candidate region. c Gene structure of Gh_D06G1908. Black rectangles and black lines represent exons and introns, respectively. d Box plots of FS and FL based on the SNP allele i11316Gh. The significance levels of the differences were assessed using two-tailed t-tests. e RT-qPCR of Gh_D06G1908. GhHis3 was used as a housekeeping gene. Error bars represent the standard deviations of three independent biological replicates